SrasV1, Main, Exploration, bibRecord, 002029

The SARS-CoV heptad repeat 2 exhibits pH-induced helix formation.

Identifieur interne : 002029 ( Main/Exploration ); précédent : 002028; suivant : 002030

The SARS-CoV heptad repeat 2 exhibits pH-induced helix formation.

Auteurs : Jessica Celigoy [États-Unis] ; Susanna Mcreynolds ; Michael Caffrey

Source :

Biochemical and biophysical research communications [ 1090-2104 ] ; 2011.

RBID : pubmed:21835164

Descripteurs français

KwdFr :
- Concentration en ions d'hydrogène, Dichroïsme circulaire, Pénétration virale, Structure secondaire des protéines, Séquences répétées d'acides aminés, Virus du SRAS (), Électricité statique.
MESH :
- Concentration en ions d'hydrogène, Dichroïsme circulaire, Pénétration virale, Structure secondaire des protéines, Séquences répétées d'acides aminés, Virus du SRAS, Électricité statique.

English descriptors

KwdEn :
- Circular Dichroism, Hydrogen-Ion Concentration, Protein Structure, Secondary, Repetitive Sequences, Amino Acid, SARS Virus (chemistry), Static Electricity, Virus Internalization.
MESH :
- chemistry : SARS Virus.
- Circular Dichroism, Hydrogen-Ion Concentration, Protein Structure, Secondary, Repetitive Sequences, Amino Acid, Static Electricity, Virus Internalization.

Abstract

The heptad repeats 1 and 2 of SARS-CoV spike, termed HR1 and HR2, play critical roles in viral entry. Moreover, HR1 and HR2 derived free peptides are inhibitors of SARS-CoV entry. In this work we used circular dichroism to show that HR2 helix formation is induced at pH 5, the pH of the endosome. In addition, we demonstrate that the HR2 helix is further stabilized at physiological ionic strengths. Together, these observations provide new insight into the mechanism of SARS-CoV entry and suggest that HR2 may be an attractive target for therapeutic intervention.

DOI: 10.1016/j.bbrc.2011.07.126
PubMed: 21835164

Affiliations:

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<affiliation wicri:level="4"><nlm:affiliation>Department of Biochemistry & Molecular Genetics University of Illinois at Chicago, Chicago, IL 60607, USA.</nlm:affiliation>
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<wicri:regionArea>Department of Biochemistry & Molecular Genetics University of Illinois at Chicago, Chicago, IL 60607</wicri:regionArea>
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<orgName type="university">Université de l'Illinois à Chicago</orgName>
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<term>Pénétration virale</term>
<term>Structure secondaire des protéines</term>
<term>Séquences répétées d'acides aminés</term>
<term>Virus du SRAS ()</term>
<term>Électricité statique</term>
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<term>Static Electricity</term>
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<term>Dichroïsme circulaire</term>
<term>Pénétration virale</term>
<term>Structure secondaire des protéines</term>
<term>Séquences répétées d'acides aminés</term>
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<front><div type="abstract" xml:lang="en">The heptad repeats 1 and 2 of SARS-CoV spike, termed HR1 and HR2, play critical roles in viral entry. Moreover, HR1 and HR2 derived free peptides are inhibitors of SARS-CoV entry. In this work we used circular dichroism to show that HR2 helix formation is induced at pH 5, the pH of the endosome. In addition, we demonstrate that the HR2 helix is further stabilized at physiological ionic strengths. Together, these observations provide new insight into the mechanism of SARS-CoV entry and suggest that HR2 may be an attractive target for therapeutic intervention.</div>
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The SARS-CoV heptad repeat 2 exhibits pH-induced helix formation.

The SARS-CoV heptad repeat 2 exhibits pH-induced helix formation.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

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